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KMID : 0352720080320030270
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Journal of Ginseng Research
2008 Volume.32 No. 3 p.270 ~ p.274
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Inhibition Effect of Ginseng Saponin on the Growth of Citrobacterer sp. Isolated from Contaminated Ginseng
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Park Chae-Kyu
Rhee Man-Hee
Kwak Yi-Seong
Hwang Mi-Sun
Hong Soon-Gi
Lee Hoon-Sang
Won Jun-Yeon
Han Gyeong-Ho
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Abstract
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A bacterium isolated from contaminated white ginseng was identified using API kit and electron microscope. This isolate was determined as rod shaped bacterium having about 1.0 μm in diameter and 2.0 to 6.0 μm in length. It had motility by peritrichous flagellum. The isolate had β-galactosidase, arginine dihydrolase and ornithin decarboxylase. It did not have ability not only to use citrate as sole carbon source and but also to produce H2S. However, it could ferment glucose, manitol, sorbitol, rhamnose, arabinose and amygdalin. From these obserbations, the isolate was identified as Citrobacter sp. Ginseng saponin was added to culture of Citrobacter sp. in order to investigate saponin’s influence on its growth. The strain was incubated at 38oC for 3 days after addition of 0.05, 0.5, 2.0 and 4.0% (w/v) of saponin, respectively and the growth rates was investigated. The relative bacterial growth inhibition rates showed 28.6, 66.7, 92.4 and 97.7%, respectively, when compared with saponin non-treated group. These results suggest that the growth of Citrobacter sp. is inhibited by saponin in a concentration-dependent manner.
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KEYWORD
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ginseng, saponin, Citrobacter sp., growth inhibition
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